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However, its practical use has been limited mainly owing to a low synthetic yield. Enzyme activity was evaluated by determining the amount of PA in control experiments prior to taking each set of measurements. The inhibition constants were determined using the Enzyme Kinetics module of the SigmaPlot 10 software (Systat Software, Inc.San Jose, CA, USA) after determining the inhibition mode by plotting the reaction velocity in the presence and absence of the inhibitor on a double-reciprocal plot.
All of the tests were conducted at least three times.